Hi Ken, A few questions: 1. What is the melting point of the paraffin you use? 2. Is there a specific reason the temperature of your water bath is 38.5 degrees? 3. Do your forceps have any type of imperfections? Bumpy, or ridged? Something that could snag on the sections? If there isn't a specific reason for the water bath temperature to be that low (cutting brain sections), I'm thinking that is playing a large part in your sticky sections. It is common to have the water bath 5 - 10 degrees below the melting point of the wax. It helps the sections stretch out from the compression that happens when being cut. Let's see what others have to say about your sticky situation. Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet<histonet@lists.utsouthwestern.edu> wrote:Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet<histonet@lists.utsouthwestern.edu> wrote: Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
