You may try to turn your water bath down a few degrees and see if this helps and what is the melting point of the paraffin you are using? Most likely a paraffin/heat situation. If I run into this issue, a quick fix is I rinse my forceps into the ice water, wipe, and the sections will not stick.
Good Luck. Ginny Kurth UWHEALTH -----Original Message----- From: Ken M via Histonet <[email protected]> Sent: Saturday, May 6, 2023 10:27 AM To: [email protected] Subject: [Histonet] Sections sticking to tweezers WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
