What is the Kaplow method? I can't find it in textbooks. A quick Google search brings up only junk papers indicating that a Kaplov method may use carcinogenic benzidine (with wrong spelling) as the chromogen. There are simple, safe and inexpensive methods for histochemical localization of sites of peroxidase activity.in tissue sections or smears of cells. Buy a textbook for about $50, read it, and save your lab a fortune in buying special kits for very simple routine histochemical jobs.
My ten cents-worth. June 2023 John Kiernan J. A. Kiernan MB, ChB, PhD, DSc Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: Kendra Beechie ND-Bismarck via Histonet <histonet@lists.utsouthwestern.edu> Sent: June 12, 2023 3:22 PM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Kendra Beechie ND-Bismarck via Histonet <histonet@lists.utsouthwestern.edu> Sent: June 12, 2023 3:22 PM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
