You could treat the decoverslipped section in 1% periodic acid (same as used in the PAS technique) for 30 minutes prior to H&E staining. This might improve the H&E staining.
I believe Lee Luna suggested this but for the life of me, I can’t find the reference! Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: jayalakshmy p.s via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Friday, 10 November 2023 3:43 AM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] Faded H&E tissue section Hello, I would like to know how to effectively restain a faded H&E tissue section. The color becomes dull when re stained. Somebody please advise. Prof. Jayalakshmy. P S _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
