Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E’s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat’s tips which he didn’t publish, but I shared them in Frieda’s 4th Ed.
Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood <afhenw...@outlook.com> wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H&E staining. This >> might improve the H&E staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can’t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children’s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet <histonet@lists.utsouthwestern.edu> >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *histonet@lists.utsouthwestern.edu >> *Subject: *[Histonet] Faded H&E tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H&E tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet