Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full
Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachert...@gmail.com marielachert...@qb.fcen.uba.ar <mariela.chert...@uab.cat> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet