I suggest asking Dr Sami Zaqout, the corresponding author of the paper you cited. A German email address is given on the left side of the first page of the free PDF file for which you provided a web link: https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/ful<https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full>. He is easy to find with Google and he is now at Qatar University. It's the most thorough description of Golgi-Cox staining that I've seen, and the pictures of results look superb. Vibratome sectioning evidently is better and quicker than embedding in nitrocellulose and cutting with a sliding microtome! The two authors' use of the word "cryoprotectant" was unfortunate in the context of a technique that does not involve freezing. John Kiernan Emeritus, Anatomy, University of Western Ontario, Canada https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Mariela Chertoff via Histonet <histonet@lists.utsouthwestern.edu> Sent: April 10, 2024 10:28 AM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Golgi-cox staining
Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachert...@gmail.com marielachert...@qb.fcen.uba.ar <mariela.chert...@uab.cat> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
