I do not recommend this. Staining over an already stained IHC can compromise both antigen integrity and staining quality due to previous antigen retrieval. We have however done this on previously stained H&E, just keep in mind you might have to run a modified protocol with 'no-depar' and run a separate slide with a positive control.
Thank you Cecilia M. Obregon -----Original Message----- From: Olson, Jennifer (Carris Lab) via Histonet <[email protected]> Sent: Tuesday, May 6, 2025 4:30 PM To: [email protected] Subject: [Histonet] staining over IHC stained slide ====================================================================================== THIS EMAIL ORIGINATED FROM OUTSIDE OF MHS. PLEASE EXERCISE CAUTION WITH ATTACHMENTS, LINKS, OR REQUESTED ACTIONS. ====================================================================================== I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. _______________________________________________ Histonet mailing list [email protected] https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LqvVzw!QjS3SG1UuL4IAqfDPuQuxeJasW20L88lnc_M-XPw4jTt217GANzqRc6CSOzG87Rqj5KIif0HM_HNhhEjzJ56_IxgDoke$ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
