Very insightful response, thank you!
Get Outlook for iOS<https://aka.ms/o0ukef> ________________________________ From: richard cartun via Histonet <[email protected]> Sent: Tuesday, May 6, 2025 5:00:33 PM To: [email protected] <[email protected]>; Olson, Jennifer (Carris Lab) <[email protected]> Subject: Re: [Histonet] staining over IHC stained slide We did this a lot when I was working at Hartford Hospital. This was especially useful when the lesion or cells of interest in the paraffin block were consumed during microtomy. The pathologist would select previously tested slides that were negative, or had minimal immunoreactivity, and then reorder the IHC test(s) in the computer, specifying which slide to use for which antibody. New labels were prepared, and we would then carefully remove the coverslip and then rehydrate to tap water. There is no need to remove the eosin (which will come out in the alcohol) or hematoxylin (which you want anyway). No need to quench endogenous peroxidase again, but you will need to perform or repeat the appropriate heat-induced epitope retrieval (HIER) when indicated. If there is immunoreactivity present in the original slide, you could re-test using a different chromogen to facilitate interpretation. Obviously, if there is no immunoreactivity in the lesion or cells of interest in the repeat s lide, and there is no internal control in the specimen to validate the negative result, the negative test result cannot be reported. Richard W. Cartun, MS, PhDMorphologic Proteomics, LLCAvon, CT USA On Tuesday, May 6, 2025 at 04:30:18 PM EDT, Olson, Jennifer (Carris Lab) via Histonet <[email protected]> wrote: I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=HKQvKuj2FgpDyQ2Txjw7zPeScXjrUkgrQ3HZD5448fo--6pvAuuFyUos2q5yhIjb&s=Uxu3ZdXA9WFE4yaLbn123OsV8H2Z7Y485KbyvfAqm-0&e= _______________________________________________ Histonet mailing list [email protected] https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=HKQvKuj2FgpDyQ2Txjw7zPeScXjrUkgrQ3HZD5448fo--6pvAuuFyUos2q5yhIjb&s=Uxu3ZdXA9WFE4yaLbn123OsV8H2Z7Y485KbyvfAqm-0&e= _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
