Correct ...... you cannot use acid decal if you want to do molecular testing.
We switched to EDTA for small biopsy specimens that needed decalcifying prior
to processing. It is also important that the specimen be adequately fixed
before decalcification. I am no longer at Hartford Hospital, but I will try to
find out where they purchase their EDTA now. Note that you cannot use EDTA for
large specimens (e.g., femoral head) because it takes way too long. You can
also carefully separate all the soft tissue fragments from bone, and process
them separately without decalcification.
Richard Cartun
On Thursday, May 29, 2025 at 04:10:03 AM EDT, MANAHIL EL BIREIR via
Histonet <[email protected]> wrote:
I hope this email finds you well.
I am reaching out regarding the optimisation of our bone marrow trephine
specimen decalcification process. Currently, we are utilizing a ready-to-use
acid rapid decalcification method. However, our Molecular Department has
observed that the acid affects the quality of molecular results.
To enhance our process and ensure optimal molecular sequencing outcomes, we
would greatly appreciate it if you could share your bone marrow trephine
decalcification protocol with us. Additionally, which decalcification reagent
you use to mitigate potential adverse effects on molecular analysis.
Kind regards,
Manahil
Sent from my iPhone
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