Hi! For "neutral" decalcification you can mix an 10-20% EDTA solution and add 4% NaOH to a pH about 7,1-7,4. There is no difference, if you use the salt or the acid, as long as the pH is checked.
We fix 3mm-thick bone trephines over night in 4% NBF, then put them for about 30 hours (8 am one day to 3 pm next day afternoon) in 20% EDTA (pH 7,2). We let it sit on a magnetic stirrer with 40°C to enhance decalcification. After rinsing in tapwater the cassettes go into paraffin-processing. Regards Gudrun -----Ursprüngliche Nachricht----- Von: MANAHIL EL BIREIR via Histonet [mailto:[email protected]] Gesendet: Donnerstag, 29. Mai 2025 10:09 An: [email protected] Betreff: [Histonet] Bone marrow trephine decalcification I hope this email finds you well. I am reaching out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results. To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis. Kind regards, Manahil Sent from my iPhone _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
