We do this often. We take the coverslip off and put the slide in xylene for at least 5 minutes. Then, we run it down to water. From the water, we load it on our Ventana ultra with the same protocol as normal but without the deparaffination step. They have an option for "wet-load". I'm sure this can be done on other platforms and manually as well.
Mehndi Helgren Lab Manager 757-664-7901 Dominion Pathology Labs. 733 Boush St. Suite 200 Norfolk, VA 23510 -----Original Message----- From: ADESUPO ADESUYI via Histonet <[email protected]> Sent: Tuesday, September 23, 2025 1:40 PM To: [email protected] Subject: [Histonet] Performing IHC stain on an H&E stained slides Hi, Does anyone has a protocol for performing IHC stain on an H&E stained slides to share? Sincerely, Ade _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
