I believe the ventana stainer does the antigen retrieval step as part of their protocol. If someone is staining by hand or has a stainer that does not do the antigen retrieval step, they will need to make sure to do it before staining.
> On Sep 24, 2025, at 7:45 AM, Histology <[email protected]> wrote: > > We do this often. We take the coverslip off and put the slide in xylene for > at least 5 minutes. Then, we run it down to water. From the water, we load > it on our Ventana ultra with the same protocol as normal but without the > deparaffination step. They have an option for "wet-load". I'm sure this can > be done on other platforms and manually as well. > > > Mehndi Helgren > Lab Manager > 757-664-7901 > Dominion Pathology Labs. > 733 Boush St. Suite 200 > Norfolk, VA 23510 > > > > -----Original Message----- > From: ADESUPO ADESUYI via Histonet <[email protected]> > Sent: Tuesday, September 23, 2025 1:40 PM > To: [email protected] > Subject: [Histonet] Performing IHC stain on an H&E stained slides > > Hi, > > Does anyone has a protocol for performing IHC stain on an H&E stained slides > to share? > > Sincerely, > Ade > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
