Hi all! Although I am rather long in the business, I came across a question, that I wanted to share with you.
With IHC-HIER one uses high-pH tris-buffers at about pH 8-9 and low-pH citrate-buffers at pH 6. Tris-buffers are sensible to changes in temperature the higher the temperature, the lower the pH. A tris-buffer of pH 8,5 at roomtemperatur should have about pH 6,5 at 98°C (if my KI-friend calculates correctly). The citrate-buffer is not affected by temperature. So as a result the actual pH in the retrieval-solution would be rather the same. That makes me thinking... Where is the difference? Why do high-pH buffer work mostly better? Am I totally wrong with my assumptions? I would be happy about any input. kind regards Gudrun Lang Gudrun Lang Biomedizinische Analytikerin Austria _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
