Hi,
I always adjust the pH of the solutions at room temperature. What
happens to the pH as the temperature changes should at least be consistent.
So as long as there is a consistent starting point and temperature change
to result in good antigen retrieval, I presume it is a reproducible process
whatever the final pH ends up being. If the end result is consistent then I
have done everything possible to maintain it.
This is more or less an acceptance of a variable that I can't really
control for. In the words of Karl Paul Reinhold Niebuhr:
"The victorious man in the day of crisis is the man who has the serenity to
accept what he cannot help and the courage to change what must be altered.
All the Best,
Amos Brooks
----------------------------------------------------------------------
> Message: 1
> Date: Sat, 11 Apr 2026 18:18:32 +0200
> From: "Gudrun Lang" <[email protected]>
> To: <[email protected]>
> Subject: [Histonet] question regarding pH in retrieval solutions
> Message-ID: <[email protected]>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi all!
>
> Although I am rather long in the business, I came across a question, that I
> wanted to share with you.
>
> With IHC-HIER one uses high-pH tris-buffers at about pH 8-9 and low-pH
> citrate-buffers at pH 6.
>
> Tris-buffers are sensible to changes in temperature ? the higher the
> temperature, the lower the pH.
>
>
>
> A tris-buffer of pH 8,5 at roomtemperatur should have about pH 6,5 at 98?C
> (if my KI-friend calculates correctly).
>
> The citrate-buffer is not affected by temperature.
>
>
>
> So as a result the actual pH in the retrieval-solution would be rather the
> same. That makes me thinking...
>
> Where is the difference? Why do high-pH buffer work mostly better? Am I
> totally wrong with my assumptions?
>
>
>
> I would be happy about any input.
>
> kind regards
>
> Gudrun Lang
>
>
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