If you investigate bleaching you could also consider converting to a different 
colour space (HSV) where the S channel represents colour saturation. I guess 
this can work as a direct measurement of the degree of bleaching. So convert to 
HSV and do histograms of the three channels separately. Be aware that the H 
channel "Hue" is a circular representation of angle, so the endpoints might not 
suit your case. You can circumvent this by shifting the values 180 degrees.

Stein

-----Original Message-----
From: Andre Briscoe <[email protected]>
Sent: 27. februar 2024 19:53
Subject: Histogram help

Hello,

I am currently working on designing a method to use ImageJ to estimate 
bleaching in corals using the histogram function and was wondering what is 
being represented in the R+G+B graph in case it is the graph best suited for 
what I am trying to do. Does anyone happen to know?

Best,
Andre Briscoe
PhD Student
NSF CREST Center for Aquatic Chemistry and Environment Institute of 
Environment, an FIU Preeminent Program


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