Hello,
I would like to segment spheroids that are in microcavities.
For the first cell, all is ok.
Here are a few lines from my macro:
run("Duplicate...", " ");
run("Invert LUT");
setAutoThreshold("Moments dark");
setOption("BlackBackground", true);
run("Convert to Mask");
run("Invert LUT");
run("Analyze Particles...", "size=6000-80000 display summarize add");For the second cell, my problem is that I'm working in white light and the edges of the wells are also detected .... One solution might be to do a threshold on the edges of the wells and the outside and remove these areas, then do a new threshold to detect spheroids, but I don't know how to write these lines of code ... :-( Thanks for any help and/or suggestions you can give me. Good day to all Patricia The images are here : https://filesender.renater.fr/?s=download&token=f580e06e-6f9a-4e4b-8696-b99037472bdc [230227_signature-mail] Toute notre actualité sur https://www.cea.fr/drf/irig et sur Biomics<https://www.bge-lab.fr/Biomics> Et sur https://twitter.com/BiomicsLab [Facebook]<https://fr-fr.facebook.com/cea.pageofficielle> [Youtube] <https://www.youtube.com/channel/UCpTMWY8NRQby8zXUKhHznfg> [Twitter] <https://twitter.com/CEA_Officiel> [LinkedIn] <https://fr.linkedin.com/company/cea> [Instagram] <https://www.instagram.com/cea_officiel/> -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
