Greetings Patricia!

"I think I can achieve what I want ... But I don't know how to go about it."

Aha!

Maybe this macro does what you like to obtain:
//
setBackgroundColor(0,0,0);
setOption("BlackBackground",true);
run("Blobs (25K)");
run("Invert LUT");
setAutoThreshold("Default dark");
run("Analyze Particles...", "add");
roiManager("Select",Array.getSequence(roiManager("size")));
roiManager("Combine");
run("Clear Outside");
resetThreshold;
roiManager("Show None");
exit();
//

Regards

Herbie

::::::::::::::::::::::::::::::::::::::::::::
Am 29.06.24 um 07:01 schrieb OBEID Patricia:
Hello everyone
I'd like to simplify some images so that I can measure the areas of interest.
Using a series of Thresholdings, I think I can achieve what I want ...
But I don't know how to go about it. In fact, with Analyse Particles, ROIs are 
generated and I would like, for example, to either remove the content of all 
the ROIs from the image or Clear Outside.
My problem is that I don't know how to select all the ROIs at the same time and 
when I try Clear Outside, everything outside the first ROI is deleted ...
With Blobs for example, I'd like to remove all the white background. Invert LUT 
/ Threshold selects all black shapes. But now I don't know what to do with the 
ROI Manager generated by Analyze Particles.
Is what I want to do possible?
If so, can any of you help me write the command lines?
Many thanks in advance for your help
Patricia


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