Hi Patricia,
Use More>>Combine in the ROI Manager and you will be able to clear outside all
the ROIs generated by the particle analyzer.
Here is an example:
run("Blobs (25K)");
setAutoThreshold("Default");
run("Analyze Particles...", "include add");
roiManager("Combine");
setBackgroundColor(0, 0, 0);
run("Clear Outside");
resetThreshold;
> On Jun 29, 2024, at 1:02 AM, OBEID Patricia <[email protected]> wrote:
>
> Hello everyone
> I'd like to simplify some images so that I can measure the areas of interest.
> Using a series of Thresholdings, I think I can achieve what I want ...
> But I don't know how to go about it. In fact, with Analyse Particles, ROIs
> are generated and I would like, for example, to either remove the content of
> all the ROIs from the image or Clear Outside.
> My problem is that I don't know how to select all the ROIs at the same time
> and when I try Clear Outside, everything outside the first ROI is deleted ...
> With Blobs for example, I'd like to remove all the white background. Invert
> LUT / Threshold selects all black shapes. But now I don't know what to do
> with the ROI Manager generated by Analyze Particles.
> Is what I want to do possible?
> If so, can any of you help me write the command lines?
> Many thanks in advance for your help
> Patricia
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