I was curious why the behavior varies for different types of ROI rotations.
For instance, this code operates exclusively on the ROI.
makeOval(81, 65, 82, 82);
run("Flip Horizontally");
run("Flip Vertically");
However, this code rotates the entire image.
makeOval(81, 65, 82, 82);
run("Rotate 90 Degrees Right");
I need to rotate the ROI can can do this by duplicating it, rotating it, and
pasting it back into the original. Also by creating an ROI that covers the
left 2/3 of the field and flipping it horizontally because central features
don't move when rotated. The goal is to create a "random" distribution with
the same density at the real sample. When coding this, I found the differences
and was curious about them.
Thank you.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, New York, NY 10016
[email protected]<mailto:[email protected]>
http://nyulmc.org/micros http://microscopynotes.com/
Cell voice (no text) 1-914-309-3270
Office 1-646-501-0567
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html
