Greetings Michael!
Did you consider:
run("Rotate...", "rotate angle=90");
Regards
Herbie
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Am 22.07.24 um 16:59 schrieb Cammer, Michael:
I was curious why the behavior varies for different types of ROI rotations.
For instance, this code operates exclusively on the ROI.
makeOval(81, 65, 82, 82);
run("Flip Horizontally");
run("Flip Vertically");
However, this code rotates the entire image.
makeOval(81, 65, 82, 82);
run("Rotate 90 Degrees Right");
I need to rotate the ROI can can do this by duplicating it, rotating it, and pasting it
back into the original. Also by creating an ROI that covers the left 2/3 of the field
and flipping it horizontally because central features don't move when rotated. The goal
is to create a "random" distribution with the same density at the real sample.
When coding this, I found the differences and was curious about them.
Thank you.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, New York, NY 10016
[email protected]<mailto:[email protected]>
http://nyulmc.org/micros http://microscopynotes.com/
Cell voice (no text) 1-914-309-3270
Office 1-646-501-0567
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