Hi Sabina, I don't know about MotionCorr but one possibility is that the images contain a specific dimension that BioFormats doesn't "know" how to handle and/or mixes up the dimensions. I'd try playing around with the dimensions order (XYZCT) in the opening panel. It also might be useful to open the metadata and look for peculiarities of the image structure. For example in Apotome imaging if the plugin doesn't recognize the phase dimension as separate series it shows similar garbled image, but I guess that Electron imaging might have even more complicated structure .
Best wishes, Stoyan Assoc.Prof. Stoyan P. Pavlov, MD, PhD Department of Anatomy and Cell biology Medical University "Prof. Dr Paraskev Stoyanov", Varna Prof. Marin Drinov Str.55 9002 Varna Bulgaria Tel: +359 (0) 52 - 677 - 068 e-mail: [email protected] [email protected] На ср, 14.08.2024 г., 17:41 ч. Sabina Musial < [email protected]> написа: > Hello, > > I have a problem that I haven't seen addressed before. I have an .mrc > electron microscopy file created as a result of motion correction using > MonionCorr2 in Relion 5. The Fiji Bio Formats plugin is not able to open it > correctly and the resulting image looks like grey noise, see attached. When > zoomed in, it seems like every column of pixels is separated by a column of > grey. I've had the same issue on a few machines, is there something I am > missing? > > Can someone please explain to me how to open .mrc files in Fiji, step by > step? > > Best wishes, > > Sabina > > [image: Screenshot 2024-08-14 at 15.23.50.png][image: Screenshot 2024-08-14 > at 15.24.12.png] > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
