Hi Sabina,

This looks like a bug in Bio-Formats to me. You can report it the
Bio-Formats team as described here:

https://bio-formats.readthedocs.io/en/v7.3.1/about/bug-reporting.html

Regards,
Curtis


On Wed, Aug 14, 2024, 09:41 Sabina Musial <
[email protected]> wrote:

> Hello,
>
> I have a problem that I haven't seen addressed before. I have an .mrc
> electron microscopy file created as a result of motion correction using
> MonionCorr2 in Relion 5. The Fiji Bio Formats plugin is not able to open it
> correctly and the resulting image looks like grey noise, see attached. When
> zoomed in, it seems like every column of pixels is separated by a column of
> grey. I've had the same issue on a few machines, is there something I am
> missing?
>
> Can someone please explain to me how to open .mrc files in Fiji, step by
> step?
>
> Best wishes,
>
> Sabina
>
> [image: Screenshot 2024-08-14 at 15.23.50.png][image: Screenshot 2024-08-14
> at 15.24.12.png]
>
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>

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