Hello Thijs, The "Port List" connection of the DIO Config.VI should be wired with an array of the digital ports you wish to use. Each port has 8 digital lines.
Unfortunately, the E-series devices do not support hardware-timed digital I/O. You could still output your points in a software loop, but you would not be able to guarantee 1000 Hz. For a card that does support this, look at the <a href="http://sine.ni.com/apps/we/nioc.vp?cid=1104&lang=US">6533</a>. For further assistance in obtaining the correct board, go to http://www.ni.com and click on "Contact NI" at the top to speak with a customer support representative. Grant M. National Instruments