Hi Keh-Ming,

Keh-Ming Wu wrote:
>  Hello,
>
> I got a problem using Mauve alignment fuction.
> I got the following error message in the console whenevere I click the
> "Align..." button in the "Align sequences..." Window:
> ----------------------------------------------------------
> OS name is: Windows XP
> Executing: 
> mauveAligner --output=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln
> --island-size=50
> --island-output=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln.islands
> --backbone-size=50 --max-backbone-gap=50
> --backbone-output=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln.backbone
> --id-matrix=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln.id_matrix
> --output-alignment=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln.alignment
> --output-guide-tree=C:\DOCUME~1\kevin\LOCALS~1\Temp\mauve15986.mln.guide_tre
> e C:\Documents and Settings\kevin\desktop\ABBdb\Mauve\ABAchr.fas
> C:\Documents and Settings\kevin\desktop\ABBdb\Mauve\ABAchr.fas.sml
> C:\Documents and Settings\kevin\desktop\ABBdb\Mauve\ABBchr.fas C:\Documents
> and Settings\kevin\desktop\ABBdb\Mauve\ABBchr.fas.sml
> Exception FileNotOpened thrown from
> Unknown()  in ..\libGenome\gnFileSource.cpp 54 Called by Unknown() Exited
> with error code: -1073741676
> ------------------------------------------------------------
> This happened in either old 1.3 or latest 2.0 version. 
> The java version is Sun's java2 plateform standard edition (build
> 1.5.0_10-b03)
>
> Could anyone help to solve such situation?
> Thanks for any clue.
>   

That error message usually means that one of the sequence files could 
not be opened; probably the file "ABAchr.fas" which supposedly lives in 
a folder called ABBdb/Mauve on your desktop. 

If ABAchr.fas is a real file, and is also in FastA format, I would 
suggest moving it (and ABBchr.fas) to another location.  Perhaps it 
would be best to create a C:\ABBdb\Mauve folder and put files there in 
order to rule out any problems with spaces in filenames.  Mauve should 
be able to handle spaces, but maybe something strange is happening on 
your windows installation...

hope that helps,
-Aaron


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