Hi,
I would agree... GST pull downs just didn't work using the antibodies I
had... a bit of 35S and it all went fine.
In our lab there is very little radioactive work and if you look at the
amounts used the risk is very low.
Take the 35S methionine i used for example: The ALI (annual limit on
intake) for this isotope is 10 mCi. I used in total for all my experiments
less than 1 mCi. As I was not eating the stuff, worked with gloves and
tried to be reasonably careful, I assume not to have incorporated any
radiation, but even if I had 'eaten' the entire aliquot I would still only
have doubled my annual background radiation, as it would not even amount to
10% ALI.
32P is of course a stronger source and actually requires adequate
shielding, but still you have a dosimeter monitoring whether or not you
have been overexposed.
In summary: I would set up a lab with radiation facilities, however
nevertheless try and reduce radioactive work where sensibly possible.
All The Best!
>
--
GRATIS für alle GMX-Mitglieder: Die maxdome Movie-FLAT!
Jetzt freischalten unter http://portal.gmx.net/de/go/maxdome01
_______________________________________________
Methods mailing list
[email protected]
http://www.bio.net/biomail/listinfo/methods
