methods  

Messages by Date

• 2019/04/03 Bionet discussion lists will be turned off this week Don Gilbert
• 2019/03/16 This Bionet discussion list will be turned off in April Don Gilbert
• 2019/02/17 Bionet at 30 years of open science communication Don Gilbert
• 2019/02/13 Stable Transfection of NIH-3T3 and 3T3-L1 sushilapthk60
• 2018/12/27 Re: Imidazole and Amicon concentrators Wolfgang Schechinger
• 2018/12/26 Imidazole and Amicon concentrators m . golgoljaam
• 2018/12/07 Re: Request for dam–/dcm– E. coli Björn Johansson
• 2018/12/06 Request for dam–/dcm– E. coli Bjorn
• 2018/10/11 Re: EGTA WS
• 2018/10/09 Re: EGTA Hiranya Roychowdhury
• 2018/10/08 Re: EGTA kkerns . mobile
• 2018/09/27 Re: How to prepare 1M H2O2 using 30% hydrogen peroxide? thekingmbembe
• 2018/08/01 Trouble with PCR saadlee shehreen
• 2018/05/06 Stock solutions of spermine/spermidine? rajkumarm574
• 2018/04/05 short oligonucleotide probe Tm and specificity analysis neda karami
• 2018/03/22 protein concentration at 280 nm Sudheer Sangeetham
• 2018/03/14 Re: Drop Dialysis of Ligations shyamals86
• 2018/02/06 Potassium Phosphate Buffer cvthakor
• 2017/11/27 Re: DNA binding proteins heathbowers
• 2017/11/26 Re: Thioester bonds travisschwochert
• 2017/11/22 Re: gc/ms for sphingosine Wolfgang Schechinger
• 2017/11/21 gc/ms for sphingosine Ed Siefker
• 2017/11/21 Re: Fwd: Slimy recombinant e.coli pellet Megha Goyal
• 2017/11/17 Need help on bioinformatics-sequence retrieval Sudheendra Rao N R
• 2017/11/16 Re: Slimy recombinant e.coli pellet P J
• 2017/11/16 Fwd: Slimy recombinant e.coli pellet Megha Goyal
• 2017/11/13 Re: pGreen Lantern sequence file Bjorn
• 2017/11/10 Re: Slimy recombinant E. coli pellet MacGregor, Barbara
• 2017/11/10 Re: Gel Solubilization Buffer Composition Hiranya Roychowdhury
• 2017/11/10 Re: Slimy recombinant e.coli pellet P J
• 2017/11/10 Re: Methods Digest, Vol 137, Issue 1 Virash Gupta
• 2017/11/10 Re: Slimy recombinant e.coli pellet Megha Goyal
• 2017/11/10 Vacuolar dye MDY-64: Chemistry? Mode of action? MacGregor, Barbara
• 2017/11/09 Re: Slimy recombinant e.coli pellet Wolfgang Schechinger
• 2017/11/09 Slimy recombinant e.coli pellet Megha Goyal
• 2017/08/05 RE: Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads Phelan, Paul J.
• 2017/08/04 Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads Sudheer Sangeetham
• 2017/07/13 Re: Phosphate-Buffered Saline precipitate when adding NaOH Dmitry Bochkariov
• 2017/07/12 Phosphate-Buffered Saline precipitate when adding NaOH najib abdellaoui
• 2017/06/15 Need a book "Liposomes: A Practical Approach" Atul Tripathi
• 2017/06/07 Re: what is the milliQ water pH? Dmitry Bochkariov
• 2017/06/07 Re: what is the milliQ water pH? Wolfgang Schechinger
• 2017/06/06 what is the milliQ water pH? Sudheer Sangeetham
• 2017/06/01 pH of urea increasing with increasing concentration Sudheer Sangeetham
• 2017/05/23 request for pFG and pFL vector map and DNA sequence. Ramachandran.B.
• 2017/05/20 Re: protein aggregation Wolfgang Schechinger
• 2017/05/19 protein aggregation TieQiao Wu
• 2017/05/17 Biocuration - Portal For Sharing Life Science Information Mohammed Iftekhar
• 2017/03/02 Re: DNA binding proteins Bjorn
• 2017/03/01 DNA binding proteins Renato De Souza Pinto Lemgruber
• 2016/12/08 Re: UV 365 nm, Photo crosslinking on ice? Dmitry Bochkariov
• 2016/12/01 Re: UV 365 nm, Photo crosslinking on ice? Hiranya Roychowdhury
• 2016/12/01 UV 365 nm, Photo crosslinking on ice? Sudheer Sangeetham
• 2016/12/01 Re: pGreen Lantern sequence file Bjorn
• 2016/11/24 Re: pGreen Lantern assembly by Bjorn Bjorn
• 2016/11/23 pGreen Lantern assembly by Bjorn MacGregor, Barbara
• 2016/11/22 pGreenLantern-1 sequence Bjorn
• 2016/11/21 pGreen Lantern-1 sequence assembly Bjorn
• 2016/11/19 pGreen Lantern, possibly helpful MacGregor, Barbara
• 2016/11/17 Re: pGreen Lantern sequence file Wolfgang Schechinger
• 2016/11/17 Re: pGreen Lantern sequence file arregui . leticia
• 2016/11/16 Simple PCR to linearize plasmid not working Phelan, Paul J.
• 2016/10/06 Re: gRNA design and off target selection Vladimir Gainullin
• 2016/10/05 gRNA design and off target selection zhyjiang2006
• 2016/09/28 C1R stable transfection Cano, Enrique
• 2016/09/21 help: plasmid extraction with unknown contamination Bing Dai
• 2016/07/31 Re: 293T cells derivative which needs medium with supplement and would not grow otherwise Christian Praetorius
• 2016/07/19 Re: Air bubbles in my agar plates mincheol . park88
• 2016/06/14 Phusion 5xbuffer m.kroiher
• 2016/05/27 Re: skim milk agar askintekin22
• 2016/05/15 skim milk agar Sue Katz
• 2016/05/13 Skim milk agar hints MacGregor, Barbara
• 2016/05/12 Query about skim milk agar sairahbano47
• 2016/05/10 293T cells derivative which needs medium with supplement and would not grow otherwise Alfredo Pagliuca
• 2016/03/10 Re: ceq 8000 lpa chovek69
• 2016/03/09 ceq 8000 lpa james jurgenson
• 2016/03/09 pcr Manali Chakraborty
• 2016/03/04 Request for software OLAIDE YEKEEN
• 2016/02/28 mitotic spreads preparation pasquale pensieri
• 2016/02/23 transient expression trouble pasquale pensieri
• 2016/02/11 Re: pGreen Lantern sequence file jjduke11
• 2016/01/19 Re: Cytofluor II (was: "Fluoroscan II software") kstumpo
• 2015/10/29 Re: the absorbance value 1.05 at 260/280 of protein solution Sudheer Sangeetham
• 2015/10/28 Re: the absorbance value 1.05 at 260/280 of protein solution Pow Joshi
• 2015/10/28 RE: the absorbance value 1.05 at 260/280 of protein solution Nair, Jayakumar
• 2015/10/28 RE: the absorbance value 1.05 at 260/280 of protein solution Phelan, Paul J.
• 2015/10/28 the absorbance value 1.05 at 260/280 of protein solution Sudheer Sangeetham
• 2015/10/25 RE: different molecular weights proteins 23 and 66 kDa? Jayakumar Nair
• 2015/10/23 RE: different molecular weights proteins 23 and 66 kDa? Nair, Jayakumar
• 2015/10/22 different molecular weights proteins 23 and 66 kDa? Sudheer Sangeetham
• 2015/10/21 Re: Dilute nitric acid for cleaning glassware and lab tools Dr S.A.D.P. Senadheera
• 2015/10/19 Dilute nitric acid for cleaning glassware and lab tools John Dudgeon
• 2015/09/30 Can I estimate the ' Fluorescent proteins' concentration at 280 nm ? Sudheer Sangeetham
• 2015/09/18 RE: Na2Po2 buffer? Hans-Georg Koenig
• 2015/09/18 Na2Po2 buffer? Sudheer Sangeetham
• 2015/09/17 Re: Cytofluor II (was: "Fluoroscan II software") gettl008
• 2015/08/31 Poly-deoxyribose phosphate? Phelan, Paul J.
• 2015/08/04 Sequncing the rest of the Gene M Aldeeb
• 2015/07/07 Re: Miscelle size of detrgent mix Dr Engelbert Buxbaum
• 2015/07/05 Miscelle size of detrgent mix Imran khan
• 2015/07/03 Re: Concentrating protein lysates Dr Engelbert Buxbaum
• 2015/07/02 kit recommendation for Baculovirus generation Mike Autry
• 2015/06/30 RE: Concentrating protein lysates Deitiker, Philip R
• 2015/06/29 Re: Concentrating protein lysates bimjhanabishwokarma
• 2015/06/23 Re: Kodak MDS 100 digital camera Sia Ghazvini
• 2015/06/23 Re: Kodak MDS 100 digital camera vivek . vasagar
• 2015/06/22 kit recommendation for Baculovirus generation Mike Autry
• 2015/06/22 Re: Home-Made TA cloning pasquale pensieri
• 2015/06/04 Re: Digestion problems with NaeI swathisuresh8666
• 2015/05/19 lentiviral construct troubleshooting pasquale pensieri
• 2015/04/29 RE: Whole cell extract gel samples for western blot Nair, Jayakumar
• 2015/04/29 Re: Whole cell extract gel samples for western blot Pow Joshi
• 2015/04/29 RNA Polymerase louhom
• 2015/04/29 Re: Whole cell extract gel samples for western blot אנה פלדמן
• 2015/04/28 Re: Whole cell extract gel samples for western blot אנה פלדמן
• 2015/04/28 Re: Whole cell extract gel samples for western blot Fulvio Celsi
• 2015/04/28 RE: Whole cell extract gel samples for western blot Alejandro Miguel Martin Dunn
• 2015/04/27 Glutathione Sepharose chromatography at below-neutral pH Phelan, Paul J.
• 2015/04/27 Whole cell extract gel samples for western blot Phelan, Paul J.
• 2015/04/14 Re: Methods Digest, Vol 114, Issue 2 Virash Gupta
• 2015/04/12 T4 DNA ligase activity in PCR buffers Bjorn
• 2015/04/01 Re: Home-Made TA cloning Pow Joshi
• 2015/04/01 RE: Home-Made TA cloning Alejandro Miguel Martin Dunn
• 2015/04/01 Re: Home-Made TA cloning anatomie . zebrafisch
• 2015/02/12 Re: co-transfection efficiency Imran khan
• 2015/02/06 co-transfection efficiency pasquale pensieri
• 2014/12/15 About PCR buffer YangJiao
• 2014/12/15 Re: PBS 1x pH dependence with time Annalisa Neri
• 2014/12/12 Re: PBS 1x pH dependence with time Dr Engelbert Buxbaum
• 2014/12/11 Methods Digest, Vol 112, Issue 2- change in pH of PBS buffer after dilution virashkgupta
• 2014/12/10 PBS 1x pH dependence with time Annalisa Neri
• 2014/12/10 Re: ibmx, dilution julipuli2003
• 2014/11/19 Re: Methods Digest, Vol 111, Issue 1 Virash Gupta
• 2014/11/17 Fwd: Request for Binary vectors/Information Kamalaker Nasani
• 2014/08/05 Storage / dilution Buffer receipe for HRP conjugates? WS
• 2014/07/25 DNA polymerases free of dNTPs? Tim Fitzwater
• 2014/07/05 Re: How much time takes to sythesis of one ATP molecule Dr Engelbert Buxbaum
• 2014/07/01 How much time takes to sythesis of one ATP molecule Sudheer Sangeetham
• 2014/06/20 Re: McIlvaine buffer WS
• 2014/06/19 Re: McIlvaine buffer ccdy81
• 2014/05/28 Re: gas chromatography ermanno . faccio
• 2014/05/25 Re: CBB R250 for microscopy Wye lab
• 2014/05/15 Re: Yellow and Orange Colonies on Kan Plates?! Christian Praetorius
• 2014/05/08 Re: Methods Digest, Vol 107, Issue 2 Virash Gupta
• 2014/05/08 RE: Yellow and Orange Colonies on Kan Plates?! Hiranya Roychowdhury
• 2014/05/06 Yellow and Orange Colonies on Kan Plates?! WS
• 2014/04/16 pTAQ licence Yannick Lequette
• 2014/04/15 Re: Methods Digest, Vol 106, Issue 7 Gerchman
• 2014/04/11 E. coli lysis protocol Theresa H
• 2014/04/11 Receipes for homemade TMB reagent (ELISA)? WS
• 2014/04/07 Re: Consort E321 Power supply specs & manuals? WS
• 2014/04/04 Protein detection tags that are dependent on proper folding somervillebikes
• 2014/04/03 Re: Consort E321 Power supply specs & manuals? Duncan Clark
• 2014/04/02 Consort E321 Power supply specs & manuals? WS
• 2014/04/02 Re: Benzoic Acid Assay WS
• 2014/04/01 RE: E coli BL21, DE3 Toufic El Arnaout
• 2014/04/01 Benzoic Acid Assay Justine Mushobozi
• 2014/03/28 Re: Publishing Equipment Twaks? Dr Engelbert Buxbaum
• 2014/03/27 RE: E coli BL21?DE3 ? Alejandro Miguel Martin Dunn
• 2014/03/27 Publishing Equipment Twaks? WS
• 2014/03/27 E coli BL21ΔDE3 ? WS
• 2014/03/25 Re: Recipe Qiagen Lysis Buffer FTB (EpiTect Plus FFPE Lysis Kit) WS
• 2014/03/25 Re: SrfI RE WS
• 2014/03/24 Recipe Qiagen Lysis Buffer FTB (EpiTect Plus FFPE Lysis Kit) Johanna Samuelsson
• 2014/03/24 Re: PCR touch and go SAID EL MOUATASSIM
• 2014/03/22 Re: hygromycin phosphotransferase Sidexcited
• 2014/03/21 Re: PCR touch and go Silky
• 2014/03/20 Re: Requesting an interview for Western Blotting Problem Dr Engelbert Buxbaum
• 2014/03/20 Re: PCR touch and go Dr Engelbert Buxbaum
• 2014/03/18 SrfI RE Karolina Malec
• 2014/03/12 PCR touch and go June May
• 2014/03/07 Requesting an interview for Western Blotting Problem RISHABH JAIN
• 2014/02/21 micro RNA Gayathri Sindhu Srinivasan
• 2014/02/13 Re: Heto FD3 freeze dryer manual jvelascoig
• 2013/12/13 comparative genomics Ed Siefker
• 2013/12/11 Re: Pharmacia / LKB FPLC System Bus - Any Hints Available? nick
• 2013/11/20 percoll solution calculations needed divya teja
• 2013/11/20 percoll solution calculations needed divya teja
• 2013/11/12 Re: tRNA cleanup/precipitation? Christian Praetorius
• 2013/11/11 Re: tRNA cleanup/precipitation? Nick Theodorakis
• 2013/11/10 Re: tRNA cleanup/precipitation? Dmitry Bochkariov
• 2013/11/06 RE: CBB R250 for microscopy Hiranya Roychowdhury
• 2013/11/06 CBB R250 for microscopy Sudheer Sangeetham
• 2013/10/24 Re: RT in-situ PCR for detecting plant viruses gdastbau
• 2013/10/15 Re: looking fo E.coli RY13 Dr Engelbert Buxbaum
• 2013/10/15 Re: Protein conformation stability test with Urea Dr Engelbert Buxbaum
• 2013/10/10 Re: looking fo E.coli RY13 tinw1988
• 2013/10/09 Protein conformation stability test with Urea Sudheer Sangeetham
• 2013/09/24 Increasing Random DNA amplification Deitiker, Philip R
• 2013/09/23 Methicillin source Duncan Clark
• 2013/09/23 photocrosslinking Sudheer Sangeetham
• 2013/08/30 Re: DNA/RNA extraction from mucinous(glycoprotein) samples WS
• 2013/08/29 Re: DNA/RNA extraction from mucinous(glycoprotein) samples Peter Ellis
• 2013/08/29 DNA/RNA extraction from mucinous(glycoprotein) samples Ed Siefker
• 2013/08/11 Re: performing statistics on normalized data Michael Sullivan
• 2013/06/03 Problems extracting DNA with magnetic beads Thomson, Neroli
• 2013/05/02 Why to use N-lauroylsarcosine for Hybridization? michel saremi
• 2013/04/26 Re: Any experiences with Geneamp 9700 cycler? Michael Sullivan
• 2013/04/26 RNA/Protein UV crosslinking (UVXL) assay Peter Ellis
• 2013/04/25 Re: Plasmid mix in the clone - the mechanism? Jonathan Rupp

• Earlier messages