Hello, I am trying to find the kd for my protein which binds GTP. I have done two sets of experiments: 1) taken constant protein and increased unlabeled GTP and monitored the tryptophan fluorescence quenching. 2) constant labeled (mant)-GTP and increased the protein and monitored the fluorescence increase with increasing protein.
I want to know if someone can help me and tell the equations (and the formulas) to feed these values in , in order to calculate the kd. Thanks Ruchi _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
