It could be hydrolysis of SDS occurring during autoclaving, producing sulfuric acid and decanol. If you can use a buffer, I highly suggest that.. The reaction that occurs is further catalyzed by the product, thus it only gets faster and faster. Hope this helps.
Josh On Fri, Dec 18, 2009 at 12:06 PM, Duncan Clark <[email protected]>wrote: > Hi Folks, > > Anyone any idea why if one autoclaves 10% (w/v) SDS prepared in MilliQ > water for 25mins instead of 15mins, it will no longer go back into solution > even when diluted 100fold into warm water? Biorad is the source a. If you > know how to get it back into solution, please tell. > > I've never come across this before. Most odd. > > Duncan > -- > I love deadlines. I especially like the whooshing noise they make as > they go flying by. > > Duncan Clark > GeneSys Ltd. > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
