Historians believe that in newspost
<[email protected]> on Mon, 21 Dec 2009,
Ed Siefker <[email protected]> penned the following literary
masterpiece:
Does anyone have any ideas? I've used these primers before
on different samples, and the melting curves always exactly
coincided, no matter what the cDNA was.
Something in one cDNA sample that isn't in the other. Could be salt, Mg,
EDTA, glycerol etc. It doesn't take much to shift 1C.
I bet if you could desalt it somehow (G25 or G50 spin column), it would
be fine.
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
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