??? Do you want soluble or insoluble (e.g. in inclusion bodies). If soluble just any his-tagged bacterial pET vector could be areasonable start. If possible, include some more tags (SUMO, MBP, GST, NusA) in parallel in your expression test and go for the one with best soluble overexpression in large-scale. Also, many proteins don`t solubly express at temperatures above 15 °C ... and others are always disappearing by degradation unless protected by coexpression of partner proteins. cheers, David
David Minde MSc (TUM) Cellular Protein Chemistry Room 707 Department of Chemistry Faculty of Science Utrecht University Krytgebouw Padualaan 8 NL-3584 CH Utrecht The Netherlands office phone +31 30 253 4105 (magic:) android +31 652614443 Science is what happens while we are making other plans (~John Lennon) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
