Quoting krishn pratap singh <[email protected]>:
Hi, Can anyone suggest me a method to purify my GST-tagged proteins from inclusion bodies. In which vector should i clone it to get the expression in soluble form. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
GE Healthcare have good handbooks on protein purification, including one on purifying "Challenging proteins", which includes a section on dealing with inclusion bodies - the pdf is here:
http://www1.gelifesciences.com/aptrix/upp00919.nsf/content/LD_367585163-R350 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
