Hi everybody I'm new to this field and I want to study whether my transcription factor (gene A) binds to the promoter of (gene B). To start with, here's is what I plan to do after doing a literature survey:
(1) Isolate chromatin from my tissue, sonicate, and IP it with an antibody that recognize my gene A (2) Design primers spanning the promoter region, enhancer region AND EXON 1 of (gene B) (3) Do a Q-PCR with chIP'd DNA as the template and primers for gene B Now my ques is; (1) How do I know whether my gene A binds to gene B. What should I expect to see when I run it on an agarose gel or do a real time Q-PCR? (2) What should be my positive and negative controls for the above expt? I would highly appreciate feedback from all the experts out there in the forum. Thanks PD _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
