Hi! In those cases, I usually prefer amplifying the fragment I need with a high fidelity polymerase rather than cutting it from a gel.
Iraz -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Senthil Thyagarajan Sent: Wednesday, January 20, 2010 11:02 AM To: methods; methods Subject: seperating 3.5 and 3.3 kb in agarosegel Dear all, I need to seperate DNA bands of size 3582bp and 3324bp and elute the former one. Can anyone suggest me the percentage of agarose gel, that could help me seperating these bands. Btw, there are no good restriction enzyme sites that break up the backbone and am left with no way other than seperating these two bands on the gel. Your help is very much appreciated. Please write to me asap. Thanks a lot in advance. Regards, Senthil _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
