hi I purified my protein under acidic conditions pH-4 with the reason that it will be free from dna. even though its 280 absorption dominates the 260 absorption the 280/260 ratio is always 1.2.
I would doubt that there is some DNA but i could not see any signal on agarose gel. I am also sure that the DNA is not homogeneous as it is overexpressed in E.coli which is not a native host. My concern is to confirm the presence or absence of DNA in the sample. I also tried Mass spectroscopy. its a failure because of some unknown reason. could you please suggest me any method that can prove the presence or absence of DNA. It would be nice if it is other than mass spectroscopy and radio labeling. -- With Regards uday ([email protected]) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
