Hi
I need to clone a pfu amplified fragment in t-vector, in the vector manual it
is said that pfu pcr product must be purified before a tailing by tag because
pfu can remove a overhangs by its proofreading activity, but in commercial high
fidelity enzyme mixes which include a proofreading enzyme with tag we can use
pcr products directly for t/a ligation, so why in the latter case A overhangs
are not removed?
regards
_______________________________________________
Methods mailing list
[email protected]
http://www.bio.net/biomail/listinfo/methods
