if you want maximum fidelity (some two orders of magnitude better than Taq), just use Phusion/Pfu ultra II fusion HS (and subsequently A-tail with Taq or subclone in blunt vector) ... just works with good yields in some 95% of cases- also with whole plasmids of some 15 kb .. Simply increases fun with clonings. cheers, David
David Minde MSc (TUM) Cellular Protein Chemistry Room 707 Department of Chemistry Faculty of Science Utrecht University Krytgebouw Padualaan 8 NL-3584 CH Utrecht The Netherlands office phone +31 30 253 4105 Science is what happens while we are making other plans (~John Lennon) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
