Hi Alex, although the scope of this NG in fact is much wider than just molecuIar biology, I doubt that it is the best group for getting a good answer. Dear fellows, I'd be happy to be proven wrong.
A quick web search like http://www.google.de/search?q=methyl+isocitrate+hplc seems to yield several promising results on the first results page. To start from scratch, I'd like to throw my 2c worth in: The actual problem should not be the separation itself, but the detection of methyl isocitrate, unless you have some sort of mass spec detector available. You'll probably need to do a pre-column derivatization: First, extract the negatively charged compounds from your analyte, e.g by ion exchange, then derivatize the carboxylic acids into something fluorescent, eg. by coupling to a fluorescent molecule with a free primary amino function (a bit like the detection of amino acids and catecholamines). Use pure (probably commercial) substances as reference. For a more direct method, you might be better off with GC after making the substance more volatile, e.g. by methylation. Detection could be with FID. Good luck! Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
