Hello there, I have a question on Em7 promoter. I am working on a BAC construct at the moment which i would use for generating a transgenic line. I have already made the targeting vector and now I need to recombine it with the BAC. But the problem is, I donot have a marker to screen for the positives, i.e, the BAC clones that carry the targeting vector in it. I dont want to use lox or Frt system, So I have decided to incorporate a EM7- neo cassette driven by a E.coli promoter EM7 at the 3' end of the gene of interest thats present in the targeting vector. This way I could screen for the BAC clones that have kanamycin resistance (the positives) in petriplates and comfortably forget about it when I am making the transgenic lines , hoping that kanamycin wont be expressed in the mammalian cells.
Any idea on these?, do anyone know how strict is this EM7 driven expression? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
