Dear All:
We recently started tissue culture work with SF9 insect cell line for protein
expression.
Since we obtained the cell line we started culturing in TC100 insect media and
then shifted to Grace's supplemented insect media. After the passage the cells
failed to continue growth and that was almost 3 weeks ago. We're not keeping
the cultures with antibiotics in order to help them grow better.
My boss obtained a second batch of cells from the firm and put them to grow
directly in Grace's media, in case the media changing might have affected the
cells irreversibly. Nevertheless, they're still not growing properly.
On the other hand, we also have many cells not attaching to the surface as
expected. Since this is actually needed to calculate baculovirus titer, I have
not started trasnfections due to these problems.
Anyone can suggest/comment on my cell culture problem?
thank you very much
Priscila
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