In <[email protected]>, Arne K Christensen <[email protected]> wrote:
> I have run dot blots with nitrocellulose membrane before; simply add > 10ul samples in RSB directly to membrane and wait for them to dry, then > block and continue with the Western blot. However, we only have PVDF > membranes on hand right now. Has anybody here run dot blots on PVDF > membrane. Do you apply the sample to the dry PVDF membrane before the > methanol soak? Will proteins bind to the membrane before the methanol soak? I have methanol soaked, then soaked in transfer buffer, than soaked in TTBS, and used it wet in the dot blotter with very very low vacuum. AC -- Email: echo 36434455860060025978157675027927670979097959886449930P | dc _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
