..aren't ladders supposed to have multiple bands? When it comes to slanting, though I haven't used acrylamide before or a precast gel, agarose gels are more likely to distort at high voltages as they heat up and start to melt. The problem is worst when using TAE, least when using sodium or lithium Borate.
But, with precast gels your choice of buffer may be limited: try a lower voltage and use a little less loading dye, see if that helps. On Mar 4, 2010 3:19 AM, <[email protected]> wrote: Hi everyone, I recently ran a 2-8% gradient polyacrylamide gel. The 1kb Plus DNA ladder had multiple bands. Does anyone know what might have caused this and how to prevent it? I suspect that I might be caused by the freeze thaw cycle. Also, some of my bands of my PCR samples were slanted using a pre-cast gel. How do you prevent that? (I washed out the wells thoroughly before use) Thanks. -Graduate student _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
