On Wed, Mar 3, 2010 at 6:23 PM, Jayakumar, R <[email protected]>wrote:
> We routinely do phosphotyrosine blots (human proteins) with > anti-phosphotyrosine antibody clone 4G10 (many companies sell this). Is it quite sensitive? Do your phosphorylated proteins bear several phospho Y sites? Could you estimate how many cells/ug protein do you load for a nice WB signal? thanks P. > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Priscila Peña Diaz > Sent: Wednesday, March 03, 2010 7:19 AM > To: [email protected] > Subject: anti-phospho S, T & Y antibodies > > Dear All: > > I've been looking recently into phosphorylation of certain membrane > proteins after a peptide antibody (specific for my protein of interest) > fails to recognize the protein without prior treatment of the membrane > (blotted > proteins) with phosphatase. > > Most of the phosphorylation assays are usually performed with labelled > phosphate (32P) but there seem to be antibodies that can also do the job . > I was wondering if anybody has experience with these monoclonal > anti-phospho S, T & Y antibodies. They look pretty fancy (and expensive) but > would like to hear if anybody has had any actual experience with them. > > kind regards > > > > Priscila > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > > This email message may contain legally privileged and/or confidential > information. If you are not the intended recipient(s), or the employee or > agent responsible for the delivery of this message to the intended > recipient(s), you are hereby notified that any disclosure, copying, > distribution, or use of this email message is prohibited. If you have > received this message in error, please notify the sender immediately by > e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
