Dear Iraz,
if I were you I would concentrate your protein using the speed-bac. This way you won't lose anything. But when you do acetone precipitation you will always lose protein, and I wouldn't use it specially if your protein concentration is very law. Hope it helps. I usually concentrate the proteins as explained. Regars Eva > From: [email protected] > To: [email protected] > Date: Thu, 11 Mar 2010 16:56:05 +0100 > CC: > Subject: Protein precipitation - acetone? > > Dear all, > > > > I have to do a western blot, but my protein concentration is very low, and I > have to run a mini gel. So I was thinking of precipitation the proteins. I > have never done this before. Does acetone have a bad effect on the blotting? > Are there any points that I should be careful about? > > > > Thanks in advance... > > > > Iraz Toprak Aydin > > > > EPFL SV ISREC, Station 19 > > Batiment SV, SV 2540 > > CH-1015 Lausanne > > Switzerland > > > > Tel: +41 21 693 07 36 > > > > e-mail: <mailto:[email protected]> [email protected] > > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods _________________________________________________________________ Escucha a quienes ya han probado Windows 7 ¡Hazlo aquí! http://www.sietesunpueblodeexpertos.com/index_windows7.html_______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
