After running a sybr green real time PCR reaction, I found that one primer set had severe primer dimers in some of my replicates, but not others.
What could cause this? I pipetted all the replicates from the same master mix (including cDNA) and the same primer mix. It seems strange to get strong dimers in one well and a perfect melt curve from the next with the exact same reagents. I'd appreciate any advice I can get. Thanks -Ed _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
