Dear Cristina Expasy <www.Expasy.ch> is a great site for DNA, RNA and protein related tools. For your specific question try <http://www.expasy.ch/tools/peptidecutter/>. Yoram
> > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 6 Apr 2010 02:02:42 -0700 (PDT) > From: LeboMad <[email protected]> > Subject: PCR failing > To: [email protected] > Message-ID: > <5a4d0356-fa5d-45ea-8b98-4b1eed90b...@u31g2000yqb.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all. I have a serious problem with PCR amplification in my lab. I > have done all troubleshooting there is out there I can't seem to get > it right. I normally get nice clear bands but for some reason I get > very faint bands if any at all. Can anyone suggest anything. I've got > a student that needs some PCR runs and needs result as in yesterday. > > > ------------------------------ > > Message: 2 > Date: Tue, 06 Apr 2010 13:05:32 +0000 > From: Christian Praetorius <[email protected]> > Subject: Re: PCR failing > To: [email protected] > Message-ID: <[email protected]> > Content-Type: text/plain; charset=us-ascii > > LeboMad <[email protected]> wrote: > > >have done all troubleshooting there is out there I can't seem to get > >it right. I normally get nice clear bands but for some reason I get > >very faint bands if any at all. Can anyone suggest anything. I've got > >a student that needs some PCR runs and needs result as in yesterday. > > The nyou should describing *exactly* what your procedure is and what > you tried to troubleshoot. > > Christian > > -- > X-no-Sig: yes > > > ------------------------------ > > Message: 3 > Date: Tue, 6 Apr 2010 15:44:52 +0200 > From: Cristina Echevarr?a Ruiz de Vargas <[email protected]> > Subject: protease cleavage site program > To: <[email protected]> > Message-ID: <eaf924ab084346398e5d96492e21f...@prmd2> > Content-Type: text/plain; charset="iso-8859-1" > > Dear colegue, > I has sow your e-mail direction associated with a program to evaluated > possibles cleavege sites in a protein. I have try to use this information but > it was not possible probably because I dont know. So I will send you a > peptide ERHHSIDAQLRALAPGKVSEE and I would ask you the possibility to look a > possible cleavege site in this peptides. Sorry for my Inglish. A would > apreciated very much if you can loock for this as I am working with a > protease that seem to attack this peptide. > > Tahnk you very much in advance > > Cristina Echevarría > > ***************************************************** > Cristina Echevarría > Profesora Titular de Universidad > Dpto. Biología Vegetla y Ecología > Facultad de Biología > Universidad de Sevilla > Sevilla > España > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 59, Issue 3 > ************************************** > ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
