Historians believe that in newspost <9de680cc-d2fe-478c-ba5d-bfbc2b53b...@u31g2000yqb.googlegroups.com> on Thu, 8 Apr 2010, LeboMad <[email protected]> penned the following literary masterpiece:
2mM dNTP

0.2mM surely? 2mM would chelate out all the Mg and it would never work.

I assume each PCR product amplifies fine in a non-multiplex and only when you multiplex there is a problem?

Primer levels used?

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
_______________________________________________
Methods mailing list
[email protected]
http://www.bio.net/biomail/listinfo/methods

Reply via email to