Cathal Garvey <[email protected]> writes: > As a possible improvement to Tom's approach, do a preceding PCR with a > much larger product by using an upstream forward primer.
Easier is to biotinylate the opposite primer, do a normal pcr with both primers, then a second reaction with only the forward primer. This will leave the template with biotin, and the ssDNA desired product with no label. Pull out the biotinylated template, leaving the desired ssDNA product pure. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
