Dear Reza, I would expect some residual activity. Isn't there any information in the papers of the people who isolated and characterized this nice enzyme?
Wo On 5 Mai, 11:37, gholamreza ahmadian <[email protected]> wrote: > Dear All > I would like to ask a question and I appreciate your any help: > I am trying to express a betagalactosidase (bgaB) from Geobacillus > stearothermophilus in E.coli. bgaB is a thermostable betagalactosidase > originally isolated from Geobacillus stearothermophilus. ATCC 7954 , glycosyl > hydrolase 42 family. The enzyme catalyses the hydrolysis of terminal, > non-reducing terminal beta-D-galactosidase residues. > It has 3 subunit from N-terminal as : 1- glycosyl hydrolase 42 family domain, > -2-non catalytic domain necessary for trimerization and -3- a domain usually > found at the C-terminus of beta-galactosidases. > > I would like to know if cloning just domain 1 (glycosyl hydrolase 42 family > domain) is functional or other domain like trimerization domain is also > necessary for functional expression of enzyme. > > Best Regards > Reza _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
