In article <[email protected]>,
[email protected] says...
>
> Hi all,
>
> Quick Western blotting question.in the past my Westerns have all been 'semi
> quantitative' where I use an image programme like ImageJ to calculate
> the intensity of the different bands. Apparently it's possible to
generate
>
> standard curves for Western blots, does anyone have any experience of
> this and can give me some general pointers?
You will probably want to read the following
@ARTICLE{Pit-07,
author = {A. Pitre and Y. Pan and S. Pruett and O. Skalli},
title = {On the use of ratio standard curves to accurately
quantitate relative changes in protein levels by western blot},
journal = {Anal. Biochem.},
volume = {361},
year = {2007},
pages = {305-307},
doi = {10.1016/j.ab.2006.11.008},
language = {engl},
}
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